Human secreted programmed cell death 1 (PD-1), also known as PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a. 25-167, with C-terminal FLAG-Avi-His-tags, MW=20 kDa, expressed in a HEK293 cell expression system. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
| 产品名称 | PD-1, FLAG-Avi-His-tag (Human) HiP™ - Soluble Receptors - Cell Surface Receptor(s) |
| 产品货号 | 71198 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 50 μg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: HEK293
MW: 20 kDa + glycans
Genbank #: NM_005018
Tag(s): C-terminal FLAG-Avi- His-tag
a.a: 25-167
Description: UniProt Q15116
Synonym(s): Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L
Assay Conditions: 10 nM PD-L1, Biotinlabeled
(BPS Cat. #71105) was mixed with
varying amounts of PD-1, FLAG-tag (BPS
Cat. #71198) in PD-1 Assay Buffer for 1 hr
at RT. FLAG-acceptor beads (left) or Nickel-acceptor
beads (right) were added and
incubated for 30 min at RT followed by
streptavidin-conjugated donor beads for 30
min at RT. Alpha counts were read.
Formulation: 8 mM phosphate pH 7.4,
110 mM NaCl, 2.2 mM KCl, and 20%
glycerol
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at -80°C
Application(s): Useful for studying protein binding and screening small molecular inhibotors.
Reference(s): 1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6. Notes: HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.
This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. Warning(s): Avoid freeze/thaw cycles. Protein may be diluted to ≥100 μg/ml in PBS + glycerol and stored at -80°C. Scientific Category: Immunotherapy
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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