The TET2 Chemiluminescent Assay Kit is designed to measure TET2 activity for screening and profiling applications. TET2 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET2 is one of the most frequently mutated genes in myeloid malignancies.

The key to the TET2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET2 activity. First, TET2 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader.

Ten-eleven translocation 2 protein, methylcytosine dioxygenase TET2

The TET2 Chemiluminescent Assay Kit comes in a convenient format; a 96-well strip plate precoated with methylated DNA substrate, primary antibody, a secondary HRP-labeled antibody, TET2 assay buffer, and purified TET2 for 100 enzyme reactions.

Catalog #	Component	Amount	Storage
50162	TET2	20 µg	-80°C	Avoid freeze/ thaw cycles!
52140Z2	Primary antibody 27	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
	4x TET2 assay buffer 1	3 ml	-80°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
	8-well strip plate module precoated with DNA substrate	1	+4°C

See assay kit data sheet for detailed protocol.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Li Tan, Yujiang Geno Shi. Development 2012; 139(11): 1895-1902.

Avoid freeze/thaw cycle

DNA Demethylase

Assay Kit