世联博研公司现货供应bpsbioscience品牌订货号为79772的STAT5 Reporter (Luc) – Ba/F3 Cell line - Cell Signaling Pathway

产品名称	STAT5 Reporter (Luc) – Ba/F3 Cell line - Cell Signaling Pathway - Cell Lines
产品货号	79772
产品价格	现货询价，电话：010-67529703
产品规格	2 vials
产品品牌	bpsbioscience
产品概述
产品详情	Description: The STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene. Materials Required But Not Provided: • mIL-3 (BPS Bioscience, #90189) • Assay Medium: Thaw Medium 8 (BPS Bioscience, #79652) • Growth Medium 8A (BPS Bioscience, #79653) • 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610) • One-Step luciferase assay system (BPS Bioscience, #60690) • Luminometer Supplied As: Each vial contains ~3 x 10^6 cells in 1 ml of FBS with 10% DMSO Host Cell line: Ba/F3: IL-3 dependent murine pro B cell line. Non-adherent cells. Mycoplasma Testing: The cell line has been screened using the PCR-based Venor^®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species. Culture Conditions: Thaw Medium 8 (BPS Bioscience, #79652) plus 5 ng/mL mouse IL-3: RPMI1640 medium (Life Technologies, #A10491-01) supplemented with 10% heat-inactivated FBS (Life Technologies, #10082147), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01), 5 ng/ml mIL-3 (BPS Bioscience, #90189). Growth Medium 8A (BPS Bioscience, #79653) plus 5 ng/mL mouse IL-3: Thaw Medium 8 (BPS Bioscience, #79652), 1 μg/ml of Puromycin (Takara, #631306), 5 ng/ml mIL-3 (BPS Bioscience, #90189). Instructions for use: See assay protocol for detailed instructions. Storage / Stability: Immediately upon receipt, store in liquid nitrogen. Application(s): • Monitor STAT5 signaling pathway activity• Screen for activators or inhibitors of STAT5 signaling pathway Notes: License Disclosure Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Cell Signaling Pathway Product Type: Cell Line Data shown is lot-specific. Contact us for specific information on other lots. Qty: Quality Assurance Product Datasheets 79772.pdf
产品资料

Quality Assurance

Product Datasheets