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产品名称 STAT3 Reporter Kit (STAT3 Signaling Pathway) - Cell Signaling Pathway - Cell Lines
产品货号 79730
产品价格 现货询价，电话：010-67529703
产品规格 500 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Background:
STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor which is activated through phosphorylation at Tyrosine 705 in response to various cytokines and growth factors. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1 and the dimers translocated to the nucleus where DNA binding/promoter induction occurs. STAT3 plays an important role in cellular processes such as cell cycle progression and apoptosis.
Description:

The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency.


The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

Materials Required But Not Provided:

• Mammalian cell line and appropriate cell culture medium
• 96-well tissue culture plate or 96-well tissue culture treated white clear-bottom
assay plate (Corning #3610)
• Transfection reagent for mammalian cell line [We use Lipofectamine™ 2000
(Invitrogen #11668027). However, other transfection reagents work equally well.
• Opti-MEM I Reduced Serum Medium (Invitrogen #31985-062)
• Dual Luciferase (Firefly-Renilla) Assay System (BPS Bioscience #60683): This
system assays cells directly in growth medium. It can be used with any
luminometer. Automated injectors are not required.
• Luminometer

Synonym(s):
Acute-Phase Response Factor, APRF, ADMIO1, HIES
Format:

Component

Specification

Amount

Storage

Reporter

(Component A)

STAT3 luciferase reporter vector + constitutively expressing Renilla luciferase vector

500 ml

(60 ng DNA/ ml)

 

-20°C

Negative Control Reporter

(Component B)

Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector

500 ml

(60 ng DNA/ ml)

-20°C
Instructions for use:
See assay protocol for detailed instruction.
Application(s):
Monitor STAT3 pathway activity. Screen activators or inhibitors of the STAT3 signaling pathway. Study effects of RNAi or gene overexpression on the activity of the STAT3 pathway.
Reference(s):

1. Tian S., et al., Blood. 1994; 84(6):1760-1764.

2. Zhong, Z., et al., Science. 1994; 264(5155):95-98.

Warning(s):
Avoid freeze/thaw cylces.
Scientific Category:
Cell Signaling Pathway
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79730.pdf
产品资料