| 产品名称 | CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit - Cell Surface Receptor(s) |
| 产品货号 | 72024 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: B7-2 (CD86) signaling through CTLA4(CD152) has been shown to inhibit T
cell activation. This co-inhibitory pathway can be overactive in many tumors, enabling cancers
to escape the host’s immune system. CTLA4-blocking antibodies, including Ipilimumab (Yervoy)
and Tremelimumab, have shown clinical efficacy in treating cancer.
Description: The CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-2 interaction. The key to this kit is the high sensitivity of detection of biotin-labeled CTLA4 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, B7-2 is coated on a 96-well plate. Next, CTLA4-biotin is incubated with B7-2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader. Synonym(s): T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, Cytotoxic T-lymphocyte-associated protein 4, CD152, B72, activity assay kit, Strep HRP, streptavidin
Supplied As: This kit comes in a convenient 96- well format, with biotin-labeled CTLA4 (CD152), purified B7-2 (CD86), streptavidin-labeled HRP, and assay buffer for 100 binding reactions
Format:
Instructions for use: See assay kit data sheet for detailed protocol
Storage / Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Application(s): This kit is useful for screening for inhibitors of CTLA4 binding to B7-2.
Reference(s): 1. Ohtani, H., et al., Lab Invest. 1997; 77(3): 231-241.
2. Rovert, C., et al., N. Engl. J. Med. 2011; 364: 2517-25262. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy/Cell Surface Receptor
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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