| 产品名称 | ADCC Bioassay Effector Cell (Mouse) - Jurkat Recombinant Cell Line |
| 产品货号 | 79733 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Mouse
Description: The mouse ADCC Reporter cells (mFcγRIV /NFAT-Jurkat cells) are recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of mouse FcγRIV (NM_144559.2).
Materials Required But Not Provided: • Assay Medium 2A (BPS Bioscience, #79621): RPMI 1640 medium (Thermo Fisher, #A1049101) supplemented with 10% low IgG FBS (Thermo Fisher, #16250078), 1% Penicillin/Streptomycin (Hyclone #SV30010.01). Synonym(s): Antibody-dependent cell-mediated cytotoxicity, natural killer bioassay, NK cell bioassay
Host Cell line: NFAT-Jurkat cells
Mycoplasma Testing: This cell line has been screened using the Venor™ GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.
Growth Media: Thaw Medium 2 (BPS Cat. #60184): RPMI1640 medium (Life Technologies, #A10491-01) supplemented with 10% FBS (Life Technologies, #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).
Format: Two vials containing ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS.
Instructions for use: See data sheet for detailed instruction and assay protocol.
Storage / Stability: Store in liquid nitrogen immediately upon receipt. Do not store for long-term at -80°C or on dry ice.
Application(s): Characterize the Fc effector function of antibodies and measure ADCC activity in cellular assays.
Reference(s): Bhavin S. Parekh, et al. 2012. “Development and validation of an antibody-dependent cellmediated Warning(s): Avoid multiple freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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