The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
| 产品名称 | Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway) |
| 产品货号 | 79271 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 500 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency. The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. * Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. Synonym(s): SRE reporter, MAPK reporter, ERK reporter
Concentration: Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)
Format:
Instructions for use: See data sheet insert for detailed transfection and assay protocols.
Storage / Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)
Application(s): • Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
• Screen for activators or inhibitors of the MAPK/ERK signaling pathway. • Study effects of RNAi or gene overexpression on the activity of the MAPK/ERK pathway. Reference(s): 1. Wong, K.K. (2009) Recent developments in anti-cancer agents targeting the Ras/Raf/ MEK/ERK pathway. Recent Pat Anticancer Drug Discov. 4(1):28-35.
2. Treisman, R. (1992) The serum response element. Trends Biochem Sci. 17(10): 423-426. Notes: Note: These vectors are ready-to-use for transient transfection. They are NOT SUITABLE for transformation and amplification in bacteria.
Warning(s): Avoid freeze/thaw cycles
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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