Human TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, also known as TAF1L, GenBank Accession No. NM_153809, a.a. 1398 – 1649 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 56.1 kDa, expressed in an E. coli expression system

Q8IZX4

TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, TAF1L

TAF1L (1398-1649) protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in TAF1L assay buffer in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, and the mixture is incubated for 30 minutes at room temperature (RT). AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer) are then added. After 10 minute incubation at RT, measure Alphacounts.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glyercol

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Wang, PJ., et al., Hum. Mol. Genet. 2002 Sep 15; 11(19): 2341-2346.

Application Reference(s):
1. Benzoisoquinolinediones as Potent and Selective Inhibitors of BRPF2 and TAF1/TAF1L Bromodomains (2017)

Avoid freeze/thaw cycles.

Bromodomain

Recombinant Protein