The TAF1 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of TAF1 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing trFluor™ europium (Eu)-labeled TAF1 (BD1+BD2), dye-labeled acceptor, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

P21675

transcription initiation factor TFIID subunit 1, cell cycle gene 1 protein, HTRF

Kit comes in a convenient format with purified Europium-labeled TAF1 (BD1+BD2) protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.

COMPONENTS

See product datasheet for detailed protocol.

At least 6 months from date of receipt when stored as directed.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Filippakopoulos, P., et al., Cell 2012; 149:214.

The final concentration of DMSO should not exceed 0.5%.

Avoid freeze/thaw cycles.

Bromodomains

Assay Kits