The SMARCA2 TR-FRET Assay Kit is designed to measure the inhibition of SMARCA2 (BRM) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, SMARCA2, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

P51531

SMARCA2, BRM, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 2, AT-Rich Interaction Domain 1B, ARID1B, HTRF

COMPONENTS:

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Schiaffino-Ortega, S., et al., 2014. J. Hematol. Oncol. 7:81.

The final concentration of DMSO should not exceed 0.5%.

Avoid freeze/thaw cycles.

Bromodomain

Assay kit