The CREBBP TR-FRET Assay Kit is designed to measure the inhibition of CREBBP binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, CREBBP, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

CREB binding protein, CBP, RSTS, KAT3A, HTRF

Kit comes in a convenient format with purified CREBBP protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.

COMPONENTS:

See assay kit data sheet for detailed protocol.

At least 6 months from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Filippakopoulos, P., et al., Cell 2012; 149:214.

Product Specific Reference(s): 
1. Unzue, A., et al., J. Med. Chem. 2015. 

The final concentration of DMSO should not exceed 0.5%.

Avoid freeze/thaw cycles.

Bromodomain

Assay Kit