Human GCN5 or KAT2A containing bromodomain, GenBank Accession No. NM_021078, a.a. 727 – 837 (end) corresponding to single bromodomain with N-terminal His-tag, MW = 14.1 kDa, expressed in an E. coli expression system.

Q92830

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04 % Tween-20, 20% glycerol

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

1. Li, S., et al., J Biol Chem. 2009 Apr 3; 284(14): 9411-7
2. Santillan, D.A., et al., Cancer Res. 2006 Oct 15; 66(20): 10032-9

Application Reference:
Quantitating the specificity and selectivity of Gcn5-mediated acetylation of histone H3 (2013)

Avoid freeze/thaw cycles.

MHHHHHHLKDPDQLYTTLKNLLAQIKSHPSAW
PFMEPVKKSEAPDYYEVIRFPIDLKTMTERLRSR
YYVTRKLFVADLQRVIANCREYNPPDSEYCRCA
SALEKFFYFKLKEGGLIDK

Bromodomain

Recombinant Protein