Human Bromodomain testis-specific protein, also known as BRDT, BRD6, and CT9, GenBank Accession No. NM_207189, a.a. 22 – 138 corresponding to bromodomain 1 with N-terminal His-tag, MW = 14.9 kDa, expressed in an E. coli expression system.

Q58F21

Bromodomain testis-specific protein, BRDT, BRD6, and CT9

BRDT-BD1 (400 nM) is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) as indicated in BRD assay buffer (BPS #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® ® Ni2+ acceptor beads (Perkin Elmer) are added, followed by Alphascreen®® Streptavidin-conjugated donor beads (Perkin Elmer). After 1 hour incubation at RT, Alphacounts are measured.

50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, and 10% glycerol

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

1. Berkovits, B.D., et al., Dev. Biol. 2011 Dec 15; 360(2): 358-368.
2. Shang, E., et al., Development. 2007 Oct; 134(19): 3507-3515.

Avoid freeze/thaw cycles.

Bromodomain

Recombinant Protein