| 产品名称 | BRD4 (BD1) Inhibitor Screening Assay Kit |
| 产品货号 | 32514 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 384 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Description: The BRD4 (BD1) Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD4 bromodomain 1 (BD1) from binding to its substrate. The key to the BRD4 (BD1) Inhibitor Screening Assay Kit is the highly specific binding of the BRD4 bromodomain 1 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD4 bromodomain 1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Materials Required But Not Provided: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Synonym(s): BRD4, Bromodomain, HUNK1, MCAP, activity assay kit
Supplied As: Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified, GST-tagged BRD4 BD1 to perform a total of 384 enzyme reactions.
Contraindications: DMSO above 0.5%. Only limited amounts of DMSO can be included, as it has been shown to disrupt BRD-ligand interaction. Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Format: COMPONENTS:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s): Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
Reference(s): McBride, A.A., et al., Trends Microbiol. 2004; 12(12):527.
Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Bromodomain
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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