ChIP results obtained with the antibody directed against HP1α, ß and γ
ChIP assays were performed using NIH3T3 cells and 4 µg of the antibody directed against HP1α, ß and γ (Cat. # 25292). QPCR was performed on the immunoprecipitated DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure 1 shows the relative enrichment as compared to a no antibody negative control ChIP.

Western blot analysis using the antibody directed against HP1α, ß and γ
Western blot was performed on nuclear extracts from HeLa cells (20 µg) with the antibody against human HP1α, ß and γ (Cat. # 25292) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right.

Immunofluorescence using the antibody directed against HP1α, ß and γ
HeLa cells were stained with the antibody against HP1α, ß and γ (Cat. # 25292) and with DAPI. Cells were fixed with 4% formaldehyde for 10 min. and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α, ß, γ antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. 

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