Cross reactivity of the monoclonal antibody directed against H3K27me2/3
Dot Blot analysis was performed to test the cross reactivity of the monoclonal antibody against H3K27me2/3 (cat. # 25242) with peptides containing other modifications of histone H3. Other modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of lysines 9 and 36. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:300.

Western blot analysis using the monoclonal antibody against H3K27me2/3
Histone extracts from HeLa cells (HeLa HE, 20 μg) were analysed by Western blot using the monoclonal antibody against H3K27me2/3 (cat. # 25242) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right.

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