产品名称 Anti-RFP-Tag, Monoclonal - Antibodies
产品货号 25013
产品价格 现货询价,电话:010-67529703
产品规格 100 µg
产品品牌 bpsbioscience
产品概述
产品详情
Host Species: Mouse
Clonality: clone RF5R
Isotype: IgG1
Background:

Red fluorescent protein cloned from Discosoma coral (DsRed or drFP583) is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).

Description:

Affinity purified Anti-Red Fluorescent Protein (RFP), clone RF5R, Mouse IgG1. Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc. Purified from mouse ascites fluid. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user. Precited M.W. of RFP by Western blot is ~26 kDa.

Synonym(s):
Antibody, RFP-tag
Formulation:

1mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3

Species Reactivity:
Sea anenome (Discosoma)
Cross Reactivity:
Does not cross-react with GFP (jellyfish).
Purification:
Protein A Affinity Chromatography
Sensitivity:
Recognizes 1-10ng of purified RFP or RFP fusion proteins by ECL.
Immunogen:
Synthetic peptide conjugated to KLH, from the N terminal region of Sea anenome (Discosoma) RFP.
Format:
Aqueous buffer solution
Storage / Stability:

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.

Application(s):
WB, DB, ELISA, IP, ICC, WB.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Antibodies
Product Type:
Antibody
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

25013.pdf

产品资料