Affinity purified Monoclonal Anti-FLAG-Tag, clone FG4R, mouse IgG2b. Recognizes FLAG-tagged (DYKDDDDK) proteins. Applications: Western Blotting (1:1000 - 3000), ELISA, Immunocytochemistry, immunoprecipitation, in situ hybridization (1:500 - 2000). Optimal dilutions/concentrations should be determined by the end user.

Antibody, FLAG-tag

1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN₃

Works in human cells; predicted to work in all proteins containing the FLAG tag, regardless of species.

Protein A Affinity Chromatography

Recognizes 1-5ng of FLAG tagged proteins in wB with ECL detection.

Synthetic peptide: DYKDDDDK conjugated to KLH.

Aqueous buffer solution

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.

WB, ICC, IP, ELISA

Avoid freeze/thaw cycles.

Antibodies

Antibody