Western blot analysis using the antibody directed against HP1α
Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the antibody against human HP1α (Cat. No. 25291), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right. The western blot analysis clearly demonstrates that the HP1α antibody specifically recognizes HP1α and not HP1β and HP1γ.

Immunofluorescence using the antibody directed against HP1α
HeLa cells were stained with the antibody against HP1α (Cat. No. 25291) and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

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