Monoclonal antibody raised in mouse against histone H3 dimethylated at lysine 36 (H3K36me2), using a KLH–conjugated synthetic peptide.

Cross reactivity of the monoclonal antibody directed against H3K36me2
To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K36me2 (cat. No. 25247). The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure 1 shows a high specificity of the antibody for the peptide containing the modification of interest.

50 µg/50 µl

PBS containing 0.05% azide

Human

Protein A purified

synthetic peptide

Aqueous buffer solution

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

ChIP (1 – 5 µg/ChIP)
ELISA (1:3000)
DB (1:10,000)
WB (1:1000 – 1:2000)

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Avoid freeze/thaw cycles

Antibodies

Antibody