| 产品名称 | ELOB/ELOC/VHL Complex - PROTACs |
| 产品货号 | 100361-1 100361-2 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg 50 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: HEK293
MW: VHL: 25 kDa; ELOB: 14 kDa; ELOC: 13 kDa
Genbank #: VHL: NM_000551; ELOB: NM_007108; ELOC: NM_005648
Tag(s): VHL: N-terminal FLAG-tag; ELOB: N-terminal FLAG-tag; ELOC: N-terminal His-tag
a.a: VHL: 2-212(end); ELOB: 2-118; ELOC: 2-112(end)
Description: ELOB/ELOC/VHL complex of human ELOB (GenBank Accession No. NM_007108), a.a. 2-118 with N-terminal FLAG-tag and MW= 14 kDa; ELOC (GenBank Accession No. NM_005648), a.a. 2-112(end) with N-terminal His-tag and MW= 13; human VHL (GenBank Accession No. NM_000551) a.a. 2-212(end) with N-terminal FLAG-tag and MW= 25 kDa, individually expressed in a HEK293 expression system.
UniProt VHL: P40337; ELOB: Q15370; ELOC: Q15369
Synonym(s): VHL, ELOB, ELOC,
Supplied As: Aqueous buffer solution.
Assay Conditions: ARV-771-mediated binding of VHL BPS #100361 to BET bromodomain was assessed using A-screen technology. Various amounts of VHL were incubated in 10-ul reaction with ARV-771 and GST-BRD3(BD2) BPS #31033 at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts. Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT.
Storage / Stability: At least 6 months at –80°C.
Application(s): Useful for the study of protein degradation and PROTAC design, and selectivity profiling.
Reference(s): 1. Kamura, T., et al., Science. 1999; 284(5414): 657-661. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Ubiquitin
Product Type: Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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