The Mouse PDE2A Assay Kit is designed for identification of inhibitors of Mouse PDE2A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE2A to the binding agent.

The key to the Mouse PDE2A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE2A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE2A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Fluorescent microplate reader capable to measure fluorescence polarization.

Adjustable micropipettor and sterile tips.

1,4-Dithiothreitol (DTT) 1 M in anhydrous DMSO.

COMPONENTS:

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

1. Salpietro, V., Perez‐Dueñas B., Nakashima K., et al. A homozygous loss‐of‐function mutation in PDE2A associated to early‐onset hereditary chorea. Movement Disorders. 2018; 33(3):482-488.

2. Gomez, L., Massari, M., et al. Design and Synthesis of Novel and Selective Phosphodiesterase 2 (PDE2a) Inhibitors for the Treatment of Memory Disorders. J. Med. Chem. 2017; 60(5):2037-2051.