Human tryptophan hydroxylase 2 (TPH2) also known as neuronal tryptophan hydroxylase, GenBank Accession No. NM_173353, a.a. 151-466 (end) with an N-terminal His-tag, MW = 37 kDa, expressed in an E. coli cell expression system.

neuronal tryptophan hydroxylase, tryptophan hyrdoxylase 2

Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH2. Fluorescence signal was measured at Em360nm/Ex300nm.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 160 mM imidazole

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Zill, P., et al., Mol. Psychiatr. 2004; 9:
1030-1036.
2. Sheehan, K., et al., Mol. Psychiatr.
2005; 10: 944-949.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Metabolic Enzyme, TPH2

Recombinant Protein