T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, Genbank Accession No. NP_049658, a.a. 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli.

P04547

T4-phage beta-glucosyltransferase, UDP glucose-DNA β-glucosyltransferase, hydroxymethyl cytosine

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Aqueous buffer solution

>6 months at –80°C

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

1. Song, C.X., et al. (2011). Nat Biotechnol. 29(1): 68-72.
2. Kornberg, S.R., et al. (1961). J. Biol. Chem. 236: 1487–1493.

Avoid freeze/thaw cycles.

Metabolic Enzymes-Transferase

Recombinant Protein