Human 6-phosphofructo-2-kinase/ fructose-2,6-biphosphatase 3, also known as PFKFB3 and IPFK2, (GenBank Accession No. NM_004566), a.a. 2-520(end)) with N-terminal His-tag, MW = 60.4 kDa, expressed in an E. coli cell expression system.

Q16875

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3, PFKFB3, PFK2, IPFK2

≥28.5 pmole/min/µg.

The reaction was done in 50 mM Tris-HCl, pH 7.5, 12.5 mM MgCl2, 1 mM β-D-fructose-6-phosphate, 100 μM ATP, 2 mM DTT, 5 mM KH2PO4, and various amount of PFKFB3 in a final volume of 50 μL. The reaction was incubated at 30°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo^® Plus reagent (Promega).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 360 mM imidazole and 20% glycerol

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Cavalier, M.C., et al., Proteins 2012;80(4):1143-1153.
2. Reddy, M.M., et al., Leukemia 2012;26(3):481-489.

Avoid freeze/thaw cycles.

Metabolic Enzymes-Kinase

Recombinant Protein