Human Peroxiredoxin I (GenBank Accession No. NM_002574), full length, with N-terminal His- tag, MW=23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q06830

PRDX1

≥896 pmol/min/µg

1.88 µg of PRDX1 was incubated in 50 mM HEPES (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30°C for 5 min, followed by the addition of 0.22 mM H₂O₂. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of biochemical function, enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Maksimov,V. et al. Genetika 42 (2), 274-277 (2006)
2. Jang,H.H et al. FEBS Lett. 580 (1), 351-355 (2006)

Avoid freeze/thaw cycles.

Peroxiredoxin

Recombinant Protein