Human nicotinamide nucleotide adenylyltransferase 2, also known as NMNAT2, (GenBank Accession No. NM_015039), a.a. 2-307 (end) with N-terminal His-tag, MW = 33 kDa, expressed in an E. coli cell expression system.

nicotinamide nucleotide adenylyltransferase 2, NMNAT2

≥23,111 pmol/min/µg

T50 mM Tris/HCl, pH 7.4, 5 mM MgCl2, 0.05% Tween-20, 0.1 mM NMN, 0.1mM ATP, and NMNAT2 (3.2 – 200ng) in a final volume of 50 µL. The reaction was incubated for 30 min at 37ºC. The amount of ATP consumed was calculated using Kinase-Glo plus reagent (Promega).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3mM DTT, 375 mM imidazole

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Yan, T., et al., NCI. 2010; 56(1): 101-106.
2. Ljungberg, M. et al., Human Molecular Genetics.  2011; 21(2): 251-267.

Avoid freeze/thaw cycles.

Metabolic Enzymes-Transferase

Recombinant Protein