The NMNAT1 Inhibitor Screening Assay Kit is designed to measure NMNAT1 activity for screening and profiling applications. The NMNAT1 assay kit comes in a convenient 96-well format, with purified recombinant NMNAT1 enzyme, NMNAT1 assay buffer, NMN, and ATP sufficient for 96 enzyme reactions.

Kinase-Glo MAX (Promega, #V6071)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

nicotinamide nucleotide adenylyltransferase 1, NMNAT1

See assay kit data sheet for detailed protocol

Up to 6 months when stored as recommended.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Schweiger, M., et al. (2001) FEBS Lett. 492 (1–2): 95–100. 

Emanuelli, M., et al. (2001) J Biol Chem. 276 (1): 406–12.

Avoid freeze/thaw cycles

Metabolic Enzymes

Assay Kit