产品名称 NAMPT Inhibitor Screening Assay Kit - Metabolic Enzymes
产品货号 71276
产品价格 现货询价,电话:010-67529703
产品规格 96 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Background:
NAMPT catalyzes the formation of nicotinamide mononucleotide from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting component in the mammalian NAD biosynthesis pathway. The protein is thought to be involved in many important biological processes, including metabolism, stress response and aging.
Description:
The NAMPT Inhibitor Screening Assay Kit is designed to measure NAMPT activity for screening and profiling applications. The NAMPT assay kit comes in a convenient 96-well format, with purified recombinant NAMPT enzyme, NAMPT assay buffer, NAMPT dilution buffer, ATP, Nicotinamide, PRPP, and Ethanol sufficient for 96 enzyme reactions.
Synonym(s):
Nicotinamide phosphoribosyltransferase protein, NAMPT, PBEF1, visfatin, Pre-B Cell-Enhancing Factor, activity assay kit
Format:

Catalog #

Reagent

Amount

Storage

91004

NAMPT

50 µg

-80°C

Avoid multiple freeze/thaw cycles!

 

NAMPT dilution buffer

3 ml

-80°C

 

4x NAMPT assay buffer

2 x 750 µl

-80°C

79686

ATP (400 μM)

250 μl

-80°C

 

Nicotinamide (400 μM)

250 μl

-80°C

 

PRPP (800 μM)

250 μl

-80°C

 

Ethanol (30%)

1 ml

-80°C

79685

Low binding black microtiter 96-well plate

1

Room Temp.

 

Instructions for use:
See product datasheet for detailed assay protocol.
Storage / Stability:
Up to 6 months from date of receipt, when stored as recommended.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Ramsey, K.M., et al. (2009) Science 324: 651-654

Nakahata, Y., et al. (2009) Science 324: 654-657.

Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:

Fluorescent microplate reader

Adjustable micropipettor and sterile tips

Aluminum foil

Scientific Category:
Metabolic Enzymes
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

71276.pdf
产品资料