Human lactate dehydrogenase A (LDHA), GenBank Accession No. NM_005566), full length [2-332(end)] with N-terminal His-tag, MW= 37.5kDa, expressed in an E. coli expression system.

P00338

Lactate dehydrogenase A, LDHA, LDH

≥700 nmole/min/µg.

Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM β-NADH, 0.02% BSA, and LDHA in a volume of 200 µL. Initiate reactions with LDHA. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. Read at Abs340 using Tecan Infinite 1000.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 160 mM imidazole, 0.04% Tween-20, and 20% glycerol.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Rong Y., et al. Tumour Biol. 2013. 34(3):1523-30.
2. Tang H., et al. Biol Reprod. 2013. 88(4):96.

Avoid freeze/thaw cycles.

Metabolic Enzymes-Dehydrogenase

Recombinant Protein