Human isocitrate dehydrogenase 2, also known as IDH2, (GenBank Accession No. NM_002168), a.a. 1-452(end) with C-terminal FLAG-tag, MW = 52 kDa, expressed in a baculovirus infected Sf9 cell expression system.

P48735

Isocitrate Dehydrogenase 2, IDH2, oxalosuccinate decarboxylase

≥14,200 pmol/min/µg.

The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl₂, 200 µM DL-isocitrate, 200 µM NADP+, and various amount of IDH2 in a volume of 200 µL. Reactions were initiated with the addition of IDH2, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M^-1cm^-1.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 3 mM DTT and 10% glycerol.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Schaap, F.G., et al., Adv Anat Pathol 2013;20(1):32-38.
2. Grassian, A.R., et al., J Biol Chem 2012;287(50):42180-42194.

Avoid freeze/thaw cycles.

Metabolic Enzymes-Dehydrogenase

Recombinant Protein