Human fucokinase (GenBank Accession No. NM_145059), a.a. 2-1084(end) with N-terminal FLAG-tag, MW = 118.6 kDa, expressed in baculovirus using an Sf9 insect cell expression system.

Q8N0W3

fucokinase, FuK

≥85.3 pmole/min/µg

The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 2 mM L-Fucose, 100 μM ATP, and various amount of fucokinase in a final volume of 50 μL. The reaction was incubated at 37°C for 1 hr, and the amount of ATP consumed was calculated using Kinase-Glo Plus^® reagent (Promega).

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol, and 80 ng/µl FLAG peptide

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Hinderlich, S., et al., Biochem Biophys Res Commun. 2002 Jun 14; 294(3):650-4.
2. Zeitler, R., et al. J. Enz. Inhib. 1997 Feb; 11(4):265-73

Avoid freeze/thaw cycles.

Metabolic Enzymes-Kinase

Recombinant Protein