Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7-H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus with the Fc portion of mouse IgG2a, expressed in a HEK293 cell expression system. MW = 53 kDa.

CD274, B7 homolog 1, B7-H1, B7H1

Add 50 uL of PD-1-Biotin in assay buffer (PBS with 0.1% BSA) to the wells precoated with PD-L1 on a white maxisorp plate. Incubate the reaction mixture at room temperature for 2 hours. Wash and block wells. Then add streptavidin-HRP and incubate for 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence.

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Useful for the study of protein-protein interaction and in vitro and in vivo protein function.

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Immunotherapy

Protein