Complex of human mTOR, also known as FRAP1 (GenBank Accession No. NM_004958), a.a. 1362-end, with N-terminal FLAG-tag, MW = 137 kDa, human MLST8 (GenBank Accession No. NM_022372), a.a. 2-326 (end) with N-terminal His-tag, MW = 37 kDa, and human Raptor (GenBank Accession No. NM_020761), a.a. 2-1335 (end) with N-terminal Histag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.

P42345 (mTOR), Q9BVC4 (MLST8), Q8N122 (Raptor)

mTOR, mechanistic target of rapamycin, FRAP, FKBP-Rapamycin Associated Protein, Mammalian Target Of Rapamycin, raptor, regulatory associated protein of MTOR, Mip1, MLST8, Mammalian Lethal With SEC13 Protein 8, GBL, Gable, POP3, LST8

7.1 pmol/min/µg

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Invitrogen Zlyte Ser/Thr peptide 11) at room temperature for 1 hour.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, and 0.18 mg/ml FLAG peptide

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Toral-Barza et al., (2005) Biochem. Biophys. Res. Commun. 24, 332(1):304-10.
2. Rubio-Viqueira and Hidalgo, M. (2006) Adv. Exp. Med. Biol. 587:309-327.

Application Reference(s):
1. mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3 (2014)
2. The mechanistic target for rapamycin pathway is related to the phosphorylation score for estrogen receptor-α in human breast tumors in vivo (2014)

Avoid freeze/thaw cycles

Kinase/Serine-Threonine, Immunotherapy

Recombinant Protein