| 产品名称 | Woodchuck PD-L1 / TCR activator - CHO Recombinant Cell line - Stable Cell Lines & Cell-Based Assay Kits - Immunotherapy |
| 产品货号 | 79457 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 Vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.
Description: Recombinant CHO-K1 cells constitutively expressing woodchuck (groundhog, Marmota monax) PD-L1 (Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7- H1), GenBank accession # HQ403651) and an engineered T cell receptor (TCR) activator.
Materials Required But Not Provided:
Synonym(s): Groundhog, Marmota Monax, PD-L1, Programmed Cell Death 1 Ligand 1, CD274, B7 Homolog 1, B7-H1, TCR
Supplied As: Each vial contains ~2.5 x 10^6 cells in 1 ml of 10% DMSO.
Host Cell line: CHO
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich,#MP0025) to confirm the absence of Mycoplasma species.
Growth Media: Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01). Growth Medium 3A (BPS Bioscience #60188): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml of Geneticin (Life Technologies #11811031) and 500 μg/ml of Hygromycin B (Hyclone #SV30070.01) to ensure recombinant expression. Cells should be grown at 37°C with 5% CO2 using complete growth medium. PD-L1 /TCR activator – CHO cells should exhibit a typical cell division time of ~24 hours. Instructions for use: See data sheet for detailed culturing and assay protocol.
Storage / Stability: Immediately upon receipt, store vials in liquid nitrogen.
Application(s): • Screen for activators or inhibitors of PD-1 signaling in a cellular context
• Screen PD-L1 antibodies for binding affinity • Characterize the biological activity of PD-1 interactions with PD-L1 Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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