Human TLR9 recognizes specific unmethylated CpG DNA motifs prevalent in microbial but not vertebrate genomic DNA, leading to innate and acquired immune responses. Stimulation of TLR9 triggers a signaling cascade leading to the activation of the transcription factor NF-κB, which controls the expression of an array of inflammatory cytokine genes.
| 产品名称 | TLR9/NF-κB Reporter – HEK293 Recombinant Cell Line - Stable Cell Lines & Cell-Based Assay Kits - Immunotherapy |
| 产品货号 | 60685 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: The family of Toll-like receptors (TLRs) acts as primary sensors that detect a wide variety of microbial components and elicit innate immune responses.
Description: Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human TLR9 (Toll-like receptor 9, also been designated as CD289, a member of the toll-like receptor (TLR) family. GenBank Accession No. NM_017442).
Materials Required But Not Provided: • Assay Medium: Thaw Medium 1 (BPS Cat. #60187) • Growth Medium 1A (BPS Cat. #79528) • ODN2006 (Invivogen tlr1-2006) • 96-well tissue culture-treated white clear-bottom assay plate • One-Step luciferase assay system (BPS Bioscience #60690) or other luciferase reagents for measuring firefly luciferase activity • Luminometer Synonym(s): TLR, TLR-9
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich #MP0025) to confirm the absence of Mycoplasma species.
Growth Media: Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01). Format: Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO
Storage / Stability: Immediately upon receipt, store in liquid nitrogen.
Application(s): Screen for activators or inhibitors of TLR9 signaling in a cellular context and characterize the biological activity of TLR9 and its interactions with ligands.
Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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