Thaw Medium 3 (BPS Cat. #60186): Ham’s F-12 medium (Hyclone # SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 3B (BPS Cat. #79529): Thaw Medium 3 plus 500 μg/ml of Hygromycin B (Hyclone #SV30070.01) to ensure the recombinant expression.

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.