Cryopreserved vial (30 x 106 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from whole blood or leukapheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in FBS with 10% DMSO at a controlled rate.
| 产品名称 | Normal Human Peripheral Blood Mononuclear Cells, Frozen - Stable Cell Lines & Cell-Based Assay Kits - Immunotherapy |
| 产品货号 | 79059 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 30 million cells |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Description: Materials Required But Not Provided: Red Blood Cell Lysis buffer (if desired): If cell pellets appear pinkish/light red it is due to a small fraction of Red Blood Cells that did not isolate from the PBMC layer. If desired/necessary, these can be quickly and easily lysed while leaving the PBMC intact, by using RBC Lysis Buffer.
RBC may not interfere with downstream PMBC culture experiments, but this will depend on the assay.
Growth Media: 10% FBS in Isocove’s Modified Dulbecco’s Medium (IMDM; Lonza, #12-722) or Lymphocyte Growth Medium 3 (LGM-3; Lonza, #CC-3211)
Instructions for use: Prepare a 50 ml conical tube with 10 ml of pre-warmed medium (10% FBS in IMDM or LGM). Quickly thaw cells in a 37°C water bath with constant and slow agitation. It is important to work quickly in the following steps to ensure high cell viability and recovery. Clean the outside of the vial with 70% ethanol and immediately transfer the entire content of the tube into the medium. Gently swirl the tube and centrifuge the cell suspension at 300 x g for 10 minutes at room temperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently resuspend the cell pellet in 15-20 mL of warm medium. Centrifuge the cell suspension at 300 x g for 10 minutes at room temperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently resuspend the cell pellet in warm medium and mix by gently flicking the tube. NOTE: Up to 30% of cell loss can be expected during washing steps. Cells are now ready for use in downstream applications. Storage / Stability: Store cells at -135˚C or colder. Thawed cells should be used immediately for downstream applications. As these are primary cells, we do not recommend maintaining these cells in culture for long periods of time.
Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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