The PARP7 Chemiluminescent Activity Assay Kit is designed to measure PARP7 activity for screening and profiling applications. PARP7 is known to catalyze the NAD-dependent ADP-ribosylation. The key to the PARP7 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

poly (ADP-ribose) polymerase 7, poly(ADP-ribosyl)transferase 7, PARP7, TCDD-inducible PARP, TIPARP

The PARP7 assay kit comes in a convenient 96-well format, with purified PARP7 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

Catalog #	Reagent	Amount	Storage
80527	PARP7	40 µg	-80°C	Avoid multiple freeze/ thaw cycles!
52029	5x histone mixture	1 ml	-80°C
	Opti-PARP 10x assay mixture containing biotinylated substrate	300 µl	-80°C
80602	10x PARP assay buffer	1 ml	-20°C
79743	Blocking buffer 3	25 ml	+4°C
80611	Streptavidin-HRP	100 µl	+4°C
79670	ELISA ECL Substrate A (translucent bottle)	6 ml	+4°C
79670	ELISA ECL Substrate B (brown bottle)	6 ml	+4°C
	Max 96-well module plate	1	Room Temp.

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Avoid freeze/thaw cycles.

PARP

Assay Kit