Mouse PDE1C (GenBank Accession No. NM_001025568), full length with N-terminal GST-tag, MW= 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q64338

PDE1C, phosphodiesterase 1C

≥ 16,650 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assayed using BPS's Mouse PDE1C Assay Kit, Cat. # 61312. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE1C at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Gong S. et al., Nature 425 (6961), 917-925 (2003).
2. Spence S. et al., Biochem. J. 321 (PT 1), 157- 163 (1997).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein