Recombinant rat vascular endothelial growth factor A (VEGF-A) expressed in E. coli cells. Rat recombinant VEGF-A contains 165 amino acids and has a molecular weight of 38.7 kDa. VEGF is an important growth factor involved in both vasculogenesis and angiogenesis.

vascular endothelial growth factor A, VEGF-A, vascular permeability factor, VPF

Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 4.0 – 8.0 ng/ml.

Recombinant rat VEGF lyophilized from 10 mM Na Phosphate, pH 7.5.

Lyophilized powder

Briefly centrifuge the vial, followed by reconstitution in sterile water to a concentration of 0.1 mg/ml. This solution can then be further diluted into other aqueous buffers containing carrier protein (0.1% BSA).

The lyophilized protein is stable at room temperature for 3 weeks and for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at 2 - 8° C for up to one week, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Useful for the study of in vitro and in vivo protein function.

1. Caires, K.C., et al. Reproduction. 2009; 138: 667-677.
2. Linderholm, B.K., et al. Ann. Onc. 2009; 20: 1639-1646.

MAPTTEGEQKAHEVVKFMDVYQRSY
CRPIETLVDIFQEYPDEIEYIFKPSCVPL
MRCAGCCNDEALECVPTSESNVTMQI
MRIKPHQSQHIGEMSFLQHSRCECRP
KKDRTKPEKHCEPCSERRKHLFVQDP
QTCKCSCKNTDSRCKARQLELNERTC
RCDKPRR

Cytokines

Recombinant Protein