| 产品名称 | Noggin - Cytokine(s) |
| 产品货号 | 90256-1 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: FreeStyle 293-F cells
MW: 28 kDa
Genbank #: NM_005450
Tag(s): none
a.a: 28–232(end)
Background: Noggin is one of a group of proteins that act as secreted antagonists of BMP activity. BMPs are essential for osteogenesis and organogenesis during embryonic development, and also play a role in tissue healing in adults. Noggin inhibits BMP activity by binding to them with high affinity, blocking their ability to bind to their receptor.
Description: Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, a.a. 28-232(end), disulfide-linked homodimer. MW=~28kDa (monomer), expressed in Freestyle 293F cells.
UniProt Q13253
Synonym(s): Noggin, symphalangism 1, SYM1, synostoses (multiple) syndrome 1, SYNS1
Endotoxin Level: < 1EU per µg of Noggin as determined by the LAL method.
Biological Activity: Measured by Noggin’s ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is <20 ng/ml in the presence of 30 ng/ml of human BMP-4.
Formulation: Lyophilized from a 0.2 µm filtered 0.1 mg/ml solution of 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA
Format: Lyophilized solid
Reconstitution: Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability: ≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -20°C to -70°C for up to 6 months. Avoid freeze/thaw cycles. Application(s): Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Reference(s): 1. Tao, Y.X., et al. (2010). Indian J. Exp. Biol. 48(5):444-52.
2. Mfopou, J.K., et al. (2010). Gastroenterology. 138(7):2233- 2245. 3. Chaturvedi, G., et al. (2009). Cell Prolif. 42(4):425-33 Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Cytokine/Growth Factors
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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