Recombinant mouse VEGF (GenBank Accession No. NM_009505) is a 39 kDa disulfide-linked homodimeric protein (a.a. 27-190), consisting of two 164 amino acid polypeptide.

Q00731

Vascular endothelial growth factor 165, VEGF

≥98% by SDS-PAGE and HPLC analysis.

< 1EU per µg of VEGF as determined by the LAL method.

ED50 is less than 2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).

Lyophilized from 0.2 µm filtered 20 mM PBS solution, pH 7.0

lyophilized protein

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

≥2 years (lyophilized) at -20°C. Reconstituted solutions can be stored in undiluted aliquots at 4°C for one week or at -20°C for up to six months.

Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.

1. Lee, S., et al. Cell 130 (4), 691-703 (2007)
2. Yamamoto, H., et al. Dev. Biol. 308 (1), 44-53 (2007)

Avoid freeze/thaw cycles.

Cytokine

Recombinant Protein