Recombinant human vascular endothelial growth factor (VEGF)165 expressed in E. coli cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF165 is the most common form of VEGF. It is a 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptides.

Endotoxin content was assayed using a LAL gel clot method.Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-10.0 ng/ml.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity..

1. Reproduction. 2009; 138: 667-677.

2. Ann. Onc. 2009; 20: 1639-1646.

Cytokine

Protein