Recombinant Lymphotactin is a disulfide-linked homodimeric protein consisting of 93 amino acid residues, and migrates as an approximately 10 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human lymphotactin (XCL1) mature chain was expressed in E. coli.

P47992

XCL1, lymphotactin, C Motif Chemokine 1, lymphotaxin, SCM-1a, ATAC, LPTN, LTN

≥97% by SDS-PAGE and HPLC

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Activity was determined by the ability to induce chemotaxis of human T-cells at concentrations ranging between 50-100 ng/ml.

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.

lyophilized protein

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

1. Hedrick JA, Zlotnik A. Clin Immunol Immunopathol. 1998 Jun,87(3):218-22.
2. Volkman BF, et al. Methods Enzymol. 2009, 461:51-70.

Avoid freeze/thaw cycles

GSEVSDKR TCVSLTTQRL PVSRIKTYTI TEGSLRAVIFITKRGLKVCA DPQATWVRDV VRSMDRKSNT RNNMIQTKPT GTQQSTNTAV TLTG

Cytokine/Growth Factors

Recombinant Protein