| 产品名称 | Human Interferon-alpha 2a - Cytokine(s) |
| 产品货号 | 90158-A |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 19 kDa
Genbank #: P01563
a.a: 24–188
Background: All known subtypes of IFN-alpha show the same antiviral antiparasitic, antiproliferative activities. Human IFN-alpha is also a potent antiviral substance in murine, porcine, and bovine cell systems. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino- terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. IFN-alpha and IFN-beta are thought to bind to the same receptor. Signal transduction mechanisms elicited after binding of IFN-alpha to its receptors involves tyrosine phosphorylation of various non-receptor tyrosine kinases belonging to the Janus kinases.
Description: Recombinant Interferon alpha 2a is a disulfide-linked monomeric protein consisting of 166 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interferon-alpha mature chain was expressed in E. coli.
UniProt P01563
Synonym(s): Interferon Alpha 2a, IFN2a, IFNA, IFNalpha, IFN
Purity: ≥98% by SDS-PAGE and HPLC
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity: The activity was determined by a viral resistance assay of Human WISH cells, and was found to be in the range of 1x108 IU/mg.
Formulation: Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.
Format: lyophilized protein
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles. Reference(s): 1. J. Biol. Chem., Sep 2009, 284: 25051 - 25064.
2. J. Biol. Chem., Sep 2009, 284: 24328 - 24340. Warning(s): Avoid freeze/thaw cycles
Amino Acid Sequence: CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
Scientific Category: Cytokine/Growth Factors
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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