| 产品名称 | Human HCC-1(CCL14) - Cytokine(s) |
| 产品货号 | 90152-A |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 9 kDa
Genbank #: Q16627
a.a: 22–93
Background: Hemofiltrate C-C chemokine (HCC)-1 is a recently described monocyte chemoattractant. CCL14 (also known as HCC-1) belongs to the CC chemokine family. Its mature propeptide is a low- affinity agonist of CCR1 that is converted to a high- affinity agonist of CCR1 and CCR5 on proteolytic processing by serine proteases. Determination of the amino acid sequence of HCC-1 revealed four cysteine residues in positions characteristic of the C-C chemokine family, and comparison with the sequences of other chemokines revealed that HCC-1 was most homologous to MIP-1a. However, several functional properties of HCC-1 were atypical of chemokines. Unlike other chemokines, HCC-1 was expressed constitutively in a number of tissues and was present at high concentrations in normal human plasma. In addition, HCC-1 was reported not to be chemotactic for leukocytes.
Description: Recombinant HCC-1 is a disulfide-linked monomeric protein consisting of 73 amino acid residues and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human HCC-1 (CCL14) mature chain was expressed in E. coli.
UniProt Q16627
Synonym(s): HCC-1, CCL14
Purity: ≥98% by SDS-PAGE and HPLC
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity: Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/ml.
Formulation: Lyophilized from 0.2 µm filtered solution of 40 mM NaCl, 10 mM phosphate buffer, pH 7.0.
Format: lyophilized protein
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles. Reference(s): 1. J. Leukoc. Biol., Sep 2001, 70: 357.
2. J. Exp. Med., Aug 1998, 188: 603 - 608. Warning(s): Avoid freeze/thaw cycles
Amino Acid Sequence: TESSSRGPYHPSECCFTYTTYKIPRQRIMDYYETNSQCSKPGIVFITKRGHSVCTNPSDKWVQDYIKDMKEN
Scientific Category: Cytokine/Growth Factors
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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