| 产品名称 | Human EMAP-II - Cytokine(s) |
| 产品货号 | 90123-B |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 18 kDa
Genbank #: Q12904
a.a: 146–312
Background: EMAP-II is activated during apoptosis by cleavage of pro-EMAP through caspase-8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP-II also has anti-angiogenic properties, which are explained by its pro-apoptotic effect on endothelial cells.
Description: Recombinant EMAP-II is a disulfide-linked monomer protein consisting of 167 amino acid residues, and migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human EMAP-II mature chain was expressed in E. coli.
UniProt Q12904
Synonym(s): EMAP2, Aminoacyl TRNA Synthetase Complex-Interacting Multifunctional Protein 1, AIMP1, Endothelial Monocyte-Activating Polypeptide 2
Purity: ≥95% by SDS-PAGE and HPLC
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity: The ED50 was determined by apoptotic effect on MCF-7 cells using a concentration of 10-50 ng/ml.
Formulation: Lyophilized from 0.2 µm filtered 20 mM phosphate buffer, 130 mM NaCl solution, pH 7.5.
Format: lyophilized protein
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles. Reference(s): 1. Cardiovasc Res, Feb 2008, 77: 580 - 589.
2. J. Leukoc. Biol., May 2004, 75: 772 - 776. Warning(s): Avoid freeze/thaw cycles
Amino Acid Sequence: SKPI DVSRLDLRIG CIITARKHPD ADSLYVEEVDVGEIAPRTVV SGLVNHVPLE QMQNRMVILL CNLKPAKMRG VLSQAMVMCA SSPEKIEILA PPNGSVPGDR ITFDAFPGEP DKELNPKKKI WEQIQPDLHT NDECVATYKG VPFEVKGKGVCRAQTMSNSG IK
Scientific Category: Cytokine/Growth Factors
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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