Human CSF1R, also known as Colony stimulating factor 1 receptor, FMS, and CD115 (GenBank Accession No. BC047521), a.a. 538-end with N-terminal GST-tag, MW=75 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

P07333

FMS, Colony stimulating factor 1 receptor, CSF1R, CD115

24 pmol/min/μg

Assay buffer: 5 mM MOPS, 2.5 mM - glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate BMX with 0.25 mg/ml Poly (Glu:Tyr, 4:1) peptide substrate and [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Walter,M., et al. Mol. Biol. 367 (3), 839-847 (2007)
2. Li, J., et al. Genesis 44 (7), 328-335 (2006).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein