| 产品名称 | Human Stem Cell Factor |
| 产品货号 | 90235-B |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 18 kDa
Genbank #: P21583
a.a: 26–189
Background: SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL-7, Epo and some other growth and differentiation factors. In combination with IL-7, SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells, for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.
Description: Recombinant SCF (Kit Ligand) is a disulfide-linked monomer protein consisting of 165 amino acid residues, and migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human SCF mature chain was expressed in E. coli.
UniProt P21583
Synonym(s): Kit Ligand, SCF, Stem Cell Factor, Steel Factor, MGF, and Mast Cell Growth Factor, KITL, CD117L
Purity: ≥97% by SDS-PAGE and HPLC
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Formulation: Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.
Format: lyophilized protein
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles. Reference(s): 1. Bedell MA, et al. J Androl. 2004 Mar-Apr,25(2):188-99.
2. Heissig B, et al. Thromb Haemost. 2003 Oct,90(4):570-6. 3. Wehrle-Haller B. Pigment Cell Res. 2003 Jun,16(3):287-96. Warning(s): Avoid freeze/thaw cycles
Amino Acid Sequence: EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA
Scientific Category: Cytokine/Growth Factors
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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